Journal: Vaccines

Article Title: Construction of a Novel Infectious Clone of Recombinant Herpesvirus of Turkey Fc-126 Expressing VP2 of IBDV

doi: 10.3390/vaccines10091391

Figure Lengend Snippet: Multistep growth kinetics of HVT-VP2-09 and DEV-H5 (UL55) compared to HVT Fc-126 on CEFs. Titers of cell-associated viruses. Titers were determined by plaque forming units of 0.1 mL of samples in three independent tests. Average titers were determined at the indicated time points after infection with an MOI of 0.01. Error bars represent the standard deviations. The recombinant HVT-VP2 virus titers showed similar growth properties as the parental virus HVT Fc-126 strain ( p = 0.07 at 72 h and p = 0.08 at 84 h).

Article Snippet: The chicken embryo fibroblasts (CEFs) cells were prepared from SPF chicken embryo eggs from Beijing Merial Vital Laboratories Animal Technology Company Limited and prepared by standard method.

Techniques: Infection, Recombinant, Virus

Journal: Vaccines

Article Title: Construction of a Novel Infectious Clone of Recombinant Herpesvirus of Turkey Fc-126 Expressing VP2 of IBDV

doi: 10.3390/vaccines10091391

Figure Lengend Snippet: Western-blotting analysis of VP2 expression of HVT-VP2-09. Lysates of CEFs infected with HVT Fc-126 and HVT-VP2-09. Proteins were separated by SDS-10% polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Merck). A mixture of VP2 polyclonal rabbit antibodies was used as the primary antibody, and a 1:10,000 dilution of goat anti-rabbit IgG (Abcam) was used as the secondary antibody. Detection was performed with enhanced chemiluminescence (Sigma-Aldrich). Lane M: DNA marker (PageRuler TM Plus, Fermentas).

Article Snippet: The chicken embryo fibroblasts (CEFs) cells were prepared from SPF chicken embryo eggs from Beijing Merial Vital Laboratories Animal Technology Company Limited and prepared by standard method.

Techniques: Western Blot, Expressing, Infection, Polyacrylamide Gel Electrophoresis, Marker